http://hdl.handle.net/2328/4796 Flinders' staff research collected for reporting or funder mandate purposes. 2013-05-22T18:09:11Z http://hdl.handle.net/2328/26700 Title: Myb-binding protein 1A (MYBBP1A) is essential for early embryonic development, controls cell cycle and mitosis, and acts as a tumor suppressor Authors: Mori, Silvia; Bernardi, Rosa; Laurent, Audrey; Resnati, Massimo; Crippa, Ambra; Gabrieli, Arianna; Keough, Rebecca Anne; Gonda, Thomas J; Blasi, Francesco Abstract: MYBBP1A is a predominantly nucleolar transcriptional regulator involved in rDNA synthesis and p53 activation via acetylation. However little further information is available as to its function. Here we report that MYBBP1A is developmentally essential in the mouse prior to blastocyst formation. In cell culture, down-regulation of MYBBP1A decreases the growth rate of wild type mouse embryonic stem cells, mouse embryo fibroblasts (MEFs) and of human HeLa cells, where it also promotes apoptosis. HeLa cells either arrest at G2/M or undergo delayed and anomalous mitosis. At mitosis, MYBBP1A is localized to a parachromosomal region and gene-expression profiling shows that its down-regulation affects genes controlling chromosomal segregation and cell cycle. However, MYBBP1A down-regulation increases the growth rate of the immortalized NIH3T3 cells. Such Mybbp1a down-regulated NIH3T3 cells are more susceptible to Ras-induced transformation and cause more potent Ras-driven tumors. We conclude that MYBBP1A is an essential gene with novel roles at the pre-mitotic level and potential tumor suppressor activity. 2012-10-08T00:00:00Z http://hdl.handle.net/2328/26699 Title: The p53 codon 72 PRO/PRO genotype may be associated with initial central visual field defects in Caucasians with primary open angle glaucoma Authors: Wiggs, Janey L; Hewitt, Alex W; Fan, Bao Jian; Wang, Dan Yi; O'Brien, Colm; Realini, Anthony; Craig, Jamie E; Dimasi, David Paul; Mackey, David A; Haines, Jonathan L; Pasquale, Louis R; Figueiredo Sena, Dayse R Abstract: Background: Loss of vision in glaucoma is due to apoptotic retinal ganglion cell loss. While p53 modulates apoptosis, gene association studies between p53 variants and glaucoma have been inconsistent. In this study we evaluate the association between a p53 variant functionally known to influence apoptosis (codon 72 Pro/Arg) and the subset of primary open angle glaucoma (POAG) patients with early loss of central visual field. Methods: Genotypes for the p53 codon 72 polymorphism (Pro/Arg) were obtained for 264 POAG patients and 400 controls from the U.S. and in replication studies for 308 POAG patients and 178 controls from Australia (GIST). The glaucoma patients were divided into two groups according to location of initial visual field defect (either paracentral or peripheral). All cases and controls were Caucasian with European ancestry. Results: The p53-PRO/PRO genotype was more frequent in the U.S. POAG patients with early visual field defects in the paracentral regions compared with those in the peripheral regions or control group (p = 2.761025). We replicated this finding in the GIST cohort (p = 7.361023, and in the pooled sample (p = 6.661027) and in a meta-analysis of both the US and GIST datasets (1.361026, OR 2.17 (1.58–2.98 for the PRO allele). Conclusions: These results suggest that the p53 codon 72 PRO/PRO genotype is potentially associated with early paracentral visual field defects in primary open-angle glaucoma patients. 2012-09-26T00:00:00Z http://hdl.handle.net/2328/26688 Title: Combination of selenium and green tea improves the efficacy of chemoprevention in a rat colorectal cancer model by modulating genetic and epigenetic biomarkers Authors: Hu, Ying; Young, Graeme Paul; Margison, Geoffrey; Le Leu, Richard Kevin Abstract: Defining biomarkers of colorectal cancer prevention by dietary or chemopreventive agents and translation to human intervention studies 2013-04-01T00:00:00Z http://hdl.handle.net/2328/26686 Title: Impact of ethnicity and socio-economic status on Staphylococcus aureus bacteremia incidence and mortality: a heavy burden in Indigenous Australians Authors: Tong, Steven Y C; van Hal, Sebastian J; Einsiedel, Lloyd John; Currie, Bart John; Turnidge, John D Abstract: This prospective study identified more than 7500 Staphylococcus aureus bacteremia (SAB) episodes occurring in population of 16,453,420 resulting in a crude annual incidence of 11. 2 episodes per 100,000 population. Overall Indigenous Australians were 5 .9 times more likely to have a SAB, especially community-associated MRSA episodes (29.2 times) compared to non-Indigenous Australians. Populations in the lowest SES quintile had an increased SAB incidence compared to higher quintiles. However, there remained a disparity between Indigenous and non-Indigenous populations across all SES quintiles. Thus our study provides robust evidence that Indigenous populations and lower SES populations are at increased risk of SAB. Furthermore, standard measures of SES do not explain the disparity in rates between Indigenous and non-Indigenous populations. 2012-10-09T00:00:00Z