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Please use this identifier to cite or link to this item: http://hdl.handle.net/2328/1290

Title: Corneal graft rejection occurs despite Fas ligand expression and apoptosis of infiltrating cells
Authors: Williams, Keryn Anne
Standfield, S D
Smith, Justine R
Coster, Douglas John
Keywords: Apoptosis
Corneal Transplantation
Endothelium, Corneal
Epithelium, Corneal
Graft Rejection
Immunoenzyme Techniques
In Situ Nick-End Labeling
Ligands
Membrane Glycoproteins
Issue Date: May-2005
Publisher: BMJ Publishing Group - http://bjo.bmjjournals.com/
Citation: K Williams, S D Standfield, J R Smith and DJ Coster 2005. Corneal graft rejection occurs despite Fas ligand expression and apoptosis of infiltrating cells. 'British Journal of Ophthalmology', May, Vol 89, No 5, 632-8
Abstract: BACKGROUND/AIMS: Constitutive expression of Fas ligand (CD95L) protects the eye against cell mediated immune responses by inducing apoptosis in infiltrating Fas bearing T cells. This study was designed to examine Fas ligand expression on acutely rejecting rat corneal grafts and to investigate the kinetics of induction of apoptosis in infiltrating leucocytes. METHODS: Orthotopic penetrating corneal transplantation was performed between genetically disparate inbred rats. Fas ligand expression and the phenotype of infiltrating leucocytes were examined by immunohistochemistry. Apoptotic nuclei were visualised in sections of normal rat cornea, rejecting allografts, and time matched isografts by terminal deoxynucleotidyl transferase mediated dUTP biotin nick end labelling (TUNEL) and quantified by video image analysis. Staining with Hoechst dye 33258 was used to confirm the presence of apoptotic nuclei. RESULTS: Fas ligand was expressed on corneal endothelial and epithelial cells during acute corneal graft rejection. At all time points examined, including as early as the fifth postoperative day, the cells infiltrating both corneal isografts and allografts were TUNEL positive. By the 15th postoperative day, over 90% of all nuclei, many of which were T cells, were apoptotic. CONCLUSION: Expression of Fas ligand is not downregulated on the cornea during allograft rejection and infiltrating leucocytes in both isografts and allografts die rapidly in situ. Despite the death of the cells believed to be responsible for rejection, isografts survive indefinitely whereas allografts are irreparably damaged.
URI: http://hdl.handle.net/2328/1290
ISSN: 0007-1161
Appears in Collections:Doug Coster
Keryn Williams

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