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Please use this identifier to cite or link to this item: http://hdl.handle.net/2328/26328

Title: Quantitation of DNA methylation by melt curve analysis
Authors: Smith, Eric
Jones, Michael Emlyn
Drew, Paul Anthony
Keywords: Medicine
Public health
DNA methylation
Issue Date: 2009
Publisher: BioMed Central Ltd.
Citation: Smith, E., Jones, M.E., & Drew, P.A., 2009. Quantitation of DNA methylation by melt curve analysis. BMC Cancer, 9:123 (24 April 2009).
Abstract: We have validated a rapid, simple in-tube method to quantify methylation which is robust and reproducible, utilizes easily designed primers and does not need proprietary algorithms or software. The technique does not depend on any operator manipulation or interpretation of the melt curves, and is suitable for use in any laboratory with a real-time thermocycler. The parameters derived provide an objective description and quantitation of the methylation in a specimen, and can be used for statistical comparisons of methylation between specimens.
URI: http://hdl.handle.net/2328/26328
ISSN: 1471-2407
Appears in Collections:Flinders Prevention, Promotion and Primary Health Care

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