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Please use this identifier to cite or link to this item: http://hdl.handle.net/2328/3177

Title: Using BOX-PCR to exclude a clonal outbreak of melioidosis
Authors: Currie, Bart John
Gal, Daniel
Mayo, Mark J
Ward, Linda Maree
Godoy, Daniel
Spratt, Brian G
LiPuma, John J
Keywords: Burkholderia pseudomallei -- Classification
DNA Fingerprinting -- Methods
Melioidosis -- Diagnosis
Issue Date: 30-Jun-2007
Publisher: BioMed Central - http://www.biomedcentral.com
Citation: Currie BJ, Gal D, Mayo M, Ward L, Godoy D, Spratt B G, LiPuma JJ 2007 Using BOX-PCR to exclude a clonal outbreak of melioidosis. BMC Infectious Diseases 2007, 7:68
Abstract: Background Although melioidosis in endemic regions is usually caused by a diverse range of Burkholderia pseudomallei strains, clonal outbreaks from contaminated potable water have been described. Furthermore B. pseudomallei is classified as a CDC Group B bioterrorism agent. Ribotyping, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) have been used to identify genetically related B. pseudomallei isolates, but they are time consuming and technically challenging for many laboratories. Methods We have adapted repetitive sequence typing using a BOX A1R primer for typing B. pseudomallei and compared BOX-PCR fingerprinting results on a wide range of well-characterized B. pseudomallei isolates with MLST and PFGE performed on the same isolates. Results BOX-PCR typing compared favourably with MLST and PFGE performed on the same isolates, both discriminating between the majority of multilocus sequence types and showing relatedness between epidemiologically linked isolates from various outbreak clusters. Conclusion Our results suggest that BOX-PCR can be used to exclude a clonal outbreak of melioidosis within 10 hours of receiving the bacterial strains.
URI: http://hdl.handle.net/2328/3177
ISSN: 1471-2334
Appears in Collections:Bart Currie

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