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dc.contributor.authorWilliams, Keryn Anne
dc.contributor.authorStandfield, Scott D
dc.contributor.authorSmith, Justine R
dc.contributor.authorCoster, Douglas John
dc.date.accessioned2006-09-25T06:29:28Z
dc.date.available2006-09-25T06:29:28Z
dc.date.issued2005-05
dc.identifier.citationK Williams, S D Standfield, J R Smith and DJ Coster 2005. Corneal graft rejection occurs despite Fas ligand expression and apoptosis of infiltrating cells. 'British Journal of Ophthalmology', May, Vol 89, No 5, 632-8en
dc.identifier.issn0007-1161
dc.identifier.urihttp://hdl.handle.net/2328/1290
dc.description.abstractBACKGROUND/AIMS: Constitutive expression of Fas ligand (CD95L) protects the eye against cell mediated immune responses by inducing apoptosis in infiltrating Fas bearing T cells. This study was designed to examine Fas ligand expression on acutely rejecting rat corneal grafts and to investigate the kinetics of induction of apoptosis in infiltrating leucocytes. METHODS: Orthotopic penetrating corneal transplantation was performed between genetically disparate inbred rats. Fas ligand expression and the phenotype of infiltrating leucocytes were examined by immunohistochemistry. Apoptotic nuclei were visualised in sections of normal rat cornea, rejecting allografts, and time matched isografts by terminal deoxynucleotidyl transferase mediated dUTP biotin nick end labelling (TUNEL) and quantified by video image analysis. Staining with Hoechst dye 33258 was used to confirm the presence of apoptotic nuclei. RESULTS: Fas ligand was expressed on corneal endothelial and epithelial cells during acute corneal graft rejection. At all time points examined, including as early as the fifth postoperative day, the cells infiltrating both corneal isografts and allografts were TUNEL positive. By the 15th postoperative day, over 90% of all nuclei, many of which were T cells, were apoptotic. CONCLUSION: Expression of Fas ligand is not downregulated on the cornea during allograft rejection and infiltrating leucocytes in both isografts and allografts die rapidly in situ. Despite the death of the cells believed to be responsible for rejection, isografts survive indefinitely whereas allografts are irreparably damaged.en
dc.format.extent361816 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.publisherBMJ Publishing Group - http://bjo.bmjjournals.com/en
dc.subjectApoptosisen
dc.subjectCorneal Transplantationen
dc.subjectEndothelium, Cornealen
dc.subjectEpithelium, Cornealen
dc.subjectGraft Rejectionen
dc.subjectImmunoenzyme Techniquesen
dc.subjectIn Situ Nick-End Labelingen
dc.subjectLigandsen
dc.subjectMembrane Glycoproteinsen
dc.subject.otherAustralian Standard Research Classification 321016 Ophthalmology and Vision Scienceen
dc.titleCorneal graft rejection occurs despite Fas ligand expression and apoptosis of infiltrating cellsen
dc.typeArticleen
dc.rights.licenseIn Copyright


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