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dc.contributor.authorZhou, Jinwen
dc.contributor.authorVoelcker, Nicolas Hans
dc.contributor.authorEllis, Amanda Vera
dc.date.accessioned2012-12-14T00:23:32Z
dc.date.available2012-12-14T00:23:32Z
dc.date.issued2010
dc.identifier.citationZhou, J., Voelcker, N.H. and Ellis, A.V., 2010. Simple surface modification of poly(dimethylsiloxane) for DNA hybridization. Biomicrofluidics, 4, 046504.en
dc.identifier.issn1932-1058
dc.identifier.urihttp://hdl.handle.net/2328/26523
dc.description.abstractHere, we present a simple chemical modification of poly(dimethylsiloxane) (PDMS) by curing a mixture of 2 wt% undecylenic acid (UDA) in PDMS prepolymer on a gold-coated glass slide. This gold slide had been previously pretreated with a self-assembled hydrophilic monolayer of 3-mercaptopropionic acid (MPA). During curing of the UDA/PDMS prepolymer, the hydrophilic UDA carboxyl moieties diffuses toward the hydrophilic MPA carboxyl moieties on the gold surface. This diffusion of the UDA within the PDMS prepolymer to the surface is a direct result of surface energy minimization. Once completely cured, the PDMS is peeled off the gold substrate, thereby exposing the interfacial carboxyl groups. These groups are then available for subsequent attachment of 5'-amino terminated DNA oligonucleotides via amide linkages. Our results show that the covalently tethered oligonucleotides can successfully capture fluorescein-labeled complementary oligonucleotides via hybridization, which are visualized using fluorescence microscopy.en
dc.language.isoen
dc.publisherAmerican Institute of Physicsen
dc.subjectPhysicsen
dc.subjectPhysical chemistryen
dc.subjectPolymersen
dc.subjectFluorescence microscopyen
dc.titleSimple surface modification of poly(dimethylsiloxane) for DNA hybridizationen
dc.typeArticleen
local.contributor.authorOrcidLookupEllis, Amanda Vera: https://orcid.org/0000-0002-0053-5641en_US


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