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dc.contributor.authorHibberd, TJ
dc.contributor.authorZagorodnyuk, Vladimir Petrovich
dc.contributor.authorSpencer, Nicholas John
dc.contributor.authorBrookes, Simon Jonathan
dc.date.accessioned2013-08-26T04:52:34Z
dc.date.available2013-08-26T04:52:34Z
dc.date.issued19/07/2012
dc.date.issued2012-07-19
dc.identifier.citationHibberd, T.J., Zagorodnyuk, V.P., Spencer, N.J. and Brookes, S.J.H., 2012. Viscerofugal neurons recorded from guinea-pig colonic nerves after organ culture. Neurogastroenterology and Motility, 24(11), 1041-1051 (e548).en
dc.identifier.issn1350-1925
dc.identifier.urihttp://hdl.handle.net/2328/26988
dc.descriptionThis is the accepted version of the following article: [Hibberd, T. J., Zagorodnyuk, V. P., Spencer, N. J. and Brookes, S. J. H. (2012), Viscerofugal neurons recorded from guinea-pig colonic nerves after organ culture. Neurogastroenterology & Motility, 24: 1041–e548], which has been published in final form at [http://dx.doi.org/10.1111/j.1365-2982.2012.01979.x]. In addition, authors may also transmit, print and share copies with colleagues, provided that there is no systematic distribution of the submitted version, e.g. posting on a listserve, network or automated delivery.en
dc.description.abstractBackground Enteric viscerofugal neurons provide cholinergic synaptic inputs to prevertebral sympathetic neurons, forming reflex circuits that control motility and secretion. Extracellular recordings of identified viscerofugal neurons have not been reported. Methods Preparations of guinea pig distal colon were maintained in organotypic culture for 4-6 days (n = 12), before biotinamide tracing, immunohistochemistry, or extracellular electrophysiological recordings from colonic nerves. Key Results After 4-6 days in organ culture, calcitonin gene-related peptide and tyrosine hydroxylase immunoreactivity in enteric ganglia was depleted, and capsaicin-induced firing (0.4 micromol L-1) was not detected, indicating that extrinsic sympathetic and sensory axons degenerate in organ culture. Neuroanatomical tracing of colonic nerves revealed that viscerofugal neurons persist and increase as a proportion of surviving axons. Extracellular recordings of colonic nerves revealed ongoing action potentials. Interestingly, synchronous bursts of action potentials were seen in 10 of 12 preparations; bursts were abolished by hexamethonium, which also reduced firing rate (400 micromol L-1, P < 0.01, n = 7). DMPP (1,1-dimethyl-4-phenylpiperazinium; 10-4 mol L-1) evoked prolonged action potential discharge. Increased firing preceded both spontaneous and stretch-evoked contractions (X2 = 11.8, df = 1, P < 0.001). Firing was also modestly increased during distensions that did not evoke reflex contractions. All single units (11/11) responded to von Frey hairs (100-300 mg) in hexamethonium or Ca2+-free solution. Conclusions & Inferences Action potentials recorded from colonic nerves in organ cultured preparations originated from viscerofugal neurons. They receive nicotinic input, which coordinates ongoing burst firing. Large bursts preceded spontaneous and reflex-evoked contractions, suggesting their synaptic inputs may arise from enteric circuitry that also drives motility. Viscerofugal neurons were directly mechanosensitive to focal compression by von Frey hairs.en
dc.description.sponsorshipAustralian National Health & Medical Research Councilen
dc.language.isoen
dc.publisherWiley-Blackwellen
dc.relationhttp://purl.org/au-research/grants/nhmrc/595969en
dc.rights� 2012 Blackwell Publishing Ltden
dc.subjectOrgan culture techniquesen
dc.subjectEnteric nervous systemen
dc.subjectNeuronsen
dc.subjectElectrophysiologyen
dc.titleViscerofugal neurons recorded from guinea-pig colonic nerves after organ cultureen
dc.typeArticleen
dc.relation.grantnumberNHMRC/595969en
dc.identifier.doihttps://doi.org/10.1111/j.1365-2982.2012.01979.xen
dc.rights.holderWiley-Blackwellen
dc.rights.licenseIn Copyright
local.contributor.authorOrcidLookupBrookes, Simon Jonathan: https://orcid.org/0000-0001-5635-0876en_US


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