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dc.contributor.authorSharrad, DF
dc.contributor.authorde Vries, E
dc.contributor.authorBrookes, Simon Jonathan
dc.date.accessioned2013-08-26T04:52:34Z
dc.date.available2013-08-26T04:52:34Z
dc.date.issued17/12/2013
dc.date.issued2013-12-17
dc.identifier.citationSharrad DF, De Vries E, Brookes SJH (2013). Selective expression of α-synuclein-immunoreactivity in vesicular acetylcholine transporter-immunoreactive axons in the guinea-pig rectum and human colon.  Journal of Comparative Neurology, 521(3), 657-676.en
dc.identifier.issn0021-9967
dc.identifier.urihttp://hdl.handle.net/2328/26992
dc.descriptionThis article appeared in a journal published by Wiley-Blackwell. Under Wiley's copyright, mandated authors are not permitted to make work available in an institutional repository.en
dc.description.abstractParkinson's disease is a neurodegenerative disorder characterized by motor and nonmotor impairments, including constipation. The hallmark pathological features of Parkinson's disease are Lewy bodies and neurites, of which aggregated α-synuclein is a major constituent. Frequently, Lewy pathology is identified in the distal gut of constipated Parkinson's disease patients. The neurons that innervate the distal gut that express α-synuclein have not been identified. We used multiple-labeling immunohistochemistry and anterograde tracing to quantify which neurons projecting to the guinea pig rectum and human colon expressed α-synuclein in their axons. α-Synuclein-immunoreactivity was present in 24 ± 0.7% of somatostatin (SOM)-immunoreactive (IR) varicosities; 20 ± 4.3% of substance P (SP)-IR varicosities and 9 ± 1.3% vasoactive intestinal polypeptide (VIP)-IR varicosities in guinea pig rectal myenteric ganglia. However, α-synuclein-immunoreactivity was localized in significantly more vesicular acetylcholine transporter (VAChT)-IR varicosities (88 ± 3%, P < 0.001). Of SOM-IR, SP-IR, and VIP-IR varicosities that lacked VAChT-immunoreactivity, only 1 ± 0.3%, 0 ± 0.3%, and 0% contained α-synuclein-immunoreactivity, respectively. 71 ± 0.8% of VAChT-IR varicosities in myenteric ganglia of human colon were α-synuclein-IR. In guinea pig rectal myenteric ganglia, α-synuclein- and VAChT-immunoreactivity coexisted in 15 ± 1.4% of biotinamide-labeled extrinsic varicosities; only 1 ± 0.3% of biotinamide-labeled extrinsic varicosities contained α-synuclein-immunoreactivity without VAChT-immunoreactivity. α-Synuclein expression in axons to the distal gut correlates closely with expression of the cholinergic marker, VAChT. This is the first report of cell-selective α-synuclein expression in the nervous system. Our results suggest cholinergic neurons in the gut may be vulnerable in Parkinson's disease.en
dc.description.sponsorshipAustralian National Health & Medical Research Councilen
dc.language.isoenen_US
dc.publisherWiley-Blackwellen_US
dc.relationhttp://purl.org/au-research/grants/nhmrc/595969
dc.rightsCopyright 2012 Wiley Periodicals, Inc.
dc.subjectParkinson's disease
dc.subjectLewy pathology
dc.subjectCholinergic neurons
dc.titleSelective expression of α-synuclein-immunoreactivity in vesicular acetylcholine transporter-immunoreactive axons in the guinea-pig rectum and human colonen_US
dc.typeArticleen
dc.relation.grantnumberNHMRC/595969
dc.identifier.doihttps://doi.org/10.1002/cne.23198en
dc.rights.holderWiley-Blackwell
local.contributor.authorOrcidLookupBrookes, Simon Jonathan: https://orcid.org/0000-0001-5635-0876en_US


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