Now showing items 55-74 of 174

    • Gene therapy for corneal dystrophies and disease, where are we? 

      Williams, Keryn Anne; Klebe, Sonja (Lippincott Williams & Wilkins, 2012-07)
      We assess studies on vector systems for delivery of transgenes to the cornea that have been published over the last year, and summarise new work on the identification of specific transgenes for corneal diseases. Recent ...
    • Genetic analysis of the clusterin gene in pseudoexfoliation syndrome. 

      Sharma, Shiwani; Burdon, Kathryn Penelope; Craig, Jamie E; Hewitt, Alex W; Wang, Jie Jin; Mackey, David A; Mitchell, Paul; McMellon, Amy E (2008)
    • Genetic Association at the 9p21 Glaucoma Locus Contributes to Sex Bias in Normal-Tension Glaucoma 

      Ng, Soo Khai; Burdon, Kathryn Penelope; Fitzgerald, Jude T; Zhou, Tiger; Fogarty, Rhys Daniel; Souzeau, Emmanuelle; Landers, John; Mills, Richard Arthur; Casson, Robert J; Ridge, Bronwyn; Graham, Stuart L; Hewitt, Alex W; Mackey, David A; Healey, Paul R; Wang, Jie Jin; Mitchell, Paul; MacGregor, Stuart; Craig, Jamie E (Association for Research in Vision and Ophthalmology (ARVO), 2016)
    • Genetic association study of exfoliation syndrome identifies a protective rare variant at LOXL1 and five new susceptibility loci 

      Aung, Tin; Ozaki, Mineo; Lee, Mei Chin; Schlotzer-Schrehardt, Ursula; Thorleifsson, Gudmar; Mizoguchi, Takanori; Igo Jr, Robert P; Haripriya, Aravind; Williams, Susan E; Astakhov, Yury S; Orr, Andrew C; Burdon, Kathryn Penelope; Nakano, Satoko; Mori, Kazuhiko; Abu-Amero, Khaled; Hauser, Michael; Li, Zheng (Genome Institute Of Singapore); Prakadeeswari, Gopalakrishnan; Cooke-Bailey, Jessica N; Cherecheanu, Alina Popa; Kang, Jae Hee; Nelson, Sarah; Hayashi, Ken; Manabe, Shin-Ichi; Kazama, Shigeyasu; Zarnowski, Tomasz; Inoue, Kenji; Irkec, Murat; Coca-Prados, Miguel; Sugiyama, Kazuhisa; Jarvela, Irma; Schlottmann, Patricio; Lerner, S Fabian; Lamari, Hasnaa; Nilgun, Yildirim; Bikbov, Mukharram; Park, Ki Ho; Cha, Soon Cheol; Yamashiro, Kenji; Zenteno, Juan C; Jonas, Jost B; Kumar, Rajesh S; Perera, Shamira A; Chan, Anita S Y; Kobakhidze, Nino; George, Ronnie; Vijaya, Lingam; Do, Tan; Edward, Deepak P; Marcos, Lourdes De Juan; Pakravan, Mohammad; Moghimi, Sasan; Ideta, Ryuichi; Bach-Holm, Daniella; Kappelgaard, Per; Wirostko, Barbara; Thomas, Samuel; Gaston, Daniel; Bedard, Karen; Greer, Wenda L; Yang, Zhenglin; Chen, Xueyi; Huang, Lulin; Sang, Jinghong; Jia, Hongyan; Jia, Liyun; Qiao, Chunyan; Zhang, Hui; Liu, Xuyang; Zhao, Bowen; Wang, Ya Xing; Xu, Liang; Leruez, Stephanie; Reynier, Pascal; Chichua, George; Tabagari, Sergo; Uebe, Steffen; Zenkel, Matthias; Berner, Daniel; Mossbock, Georg; Weisschuh, Nicole; Hoja, Ursula; Welge-Luessen, Ulrich-Christoph; Mardin, Christian; Founti, Panayiota; Chatzikyriakidou, Anthi; Pappas, Theofanis; Anastasopoulos, Eleftherios; Lambropoulos, Alexandros; Ghosh, Arkasubhra; Shetty, Rohit; Porporato, Natalia; Saravanan, Vijayan; Venkatesh, Rengaraj; Shivkumar, Chandrashekaran; Kalpana, Narendran; Sarangapani, Sripriya; Kanavi, Mozhgan R; Beni, Afsaneh Naderi; Yazdani, Shahin; Lashay, Alireza; Naderifar, Homa; Khatibi, Nassim; Fea, Antonio; Lavia, Carlo; Dallorto, Laura; Rolle, Teresa; Frezzotti, Paolo; Paoli, Daniela; Salvi, Erika; Manunta, Paolo; Mori, Yosai; Miyata, Kazunori; Higashide, Tomomi; Chihara, Etsuo; Ishiko, Satoshi; Yoshida, Akitoshi; Yanagi, Masahide; Kiuchi, Yoshiaki; Ohashi, Tsutomu; Sakurai, Toshiya; Sugimoto, Takako; Chuman, Hideki; Aihara, Makoto; Inatani, Masaru; Miyake, Masahiro; Gotoh, Norimoto; Matsuda, Fumihiko; Yoshimura, Nagahisa; Ikeda, Yoko; Ueno, Morio; Sotozono, Chie; Jeoung, Jin Wook; Sagong, Min; Park, Kyu Hyung; Ahn, Jeeyun; Cruz-Aguilar, Marisa; Ezzouhairi, Sidi M; Rafei, Abderrahman; Chong, Yaan Fun; Ng, Xiao Yu; Goh, Shuang Ru; Chen, Yueming; Yong, Victor H K; Khan, Muhammad Imran; Olawoye, Olusola O; Ashaye, Adeyinka O; Ugbede, Idakwo; Onakoya, Adeola; Kizor-Akaraiwe, Nkiru; Teekhasaenee, Chaiwat; Suwan, Yanin; Supakontanasan, Wasu; Okeke, Suhanya; Uche, Nkechi J; Asimadu, Ifeoma; Ayub, Humaira; Akhtar, Farah; Kosior-Jarecka, Ewa; Lukasik, Urszula; Lischinsky, Ignacio; Castro, Vania; Grossmann, Rodolfo Perez; Megevand, Gordana Sunaric; Roy, Sylvain; Dervan, Edward; Silke, Eoin; Rao, Aparna; Sahay, Priti; Fornero, Pablo; Cuello, Osvaldo; Sivori, Delia; Zompa, Tamara; Mills, Richard Arthur; Souzeau, Emmanuelle; Mitchell, Paul; Wang, Jie Jin; Hewitt, Alex W; Coote, Michael; Crowston, Jonathan G; Astakhov, Sergei Y; Akopov, Eugeny L; Emelyanov, Anton; Vysochinskaya, Vera; Kazakbaeva, Gyulli; Fayzrakhmanov, Rinat; Al-Obeidan, Saleh A; Owaidhah, Ohoud; Aljasim, Leyla Ali; Chowbay, Balram; Foo, Jia Nee; Soh, Raphael Q; Sim, Kar Seng; Xie, Zhicheng; Cheong, Augustine W O; Mok, Shi Qi; Soo, Hui Meng; Chen, Xiao Y; Peh, Su Qin; Heng, Khai Koon; Husain, Rahat; Ho, Su-Ling; Hillmer, Axel M; Cheng, Ching-Yu; Escudero-Dominguez, F A; Gonzalez-Sarmiento, Rogelio; Martinon-Torres, Frederico; Salas, Antonio; Pathanapitoon, Kessara; Hansapinyo, Linda; Wanichwecharugruang, Boonsong; Kitnarong, Naris; Sakuntabhai, Anavaj; Nguyn, Hip X; Nguyn, Giang T T; Nguyn, Trinh V; Zenz, Werner; Binder, Alexander; Klobassa, Daniela S; Hibberd, Martin L; Davila, Sonia; Herms, Stefan; Nothen, Markus M; Moebus, Susanne; Rautenbach, Robyn M; Ziskind, Ari; Carmichael, Trevor R; Ramsay, Michele; Alvarez, Lydia; Garcia, Montserrat; Gonzalez-Iglesias, Hector; Rodriguez-Calvo, Pedro P; Fernandez-Vega Cueto, Luis; Oguz, Cilingir; Tamcelik, Nevbahar; Atalay, Eray; Batu, Bilge; Aktas, Dilek; Kasim, Burcu; Wilson, M Roy; Coleman, Anne L; Liu, Yutao; Challa, Pratap; Herndon, Leon; Kuchtey, Rachel W; Kuchtey, John (Nature Publishing Group, 2017-05-29)
      Exfoliation syndrome (XFS) is the most common known risk factor for secondary glaucoma and a major cause of blindness worldwide. Variants in two genes, LOXL1 and CACNA1A, have previously been associated with XFS. To further ...
    • Genetic pleiotropy between age-related macular degeneration and 16 complex diseases and traits 

      Grassmann, F; Kiel, C; Zimmermann, M E; Gorski, M; Grassmann, V; Stark, K; International AMD Genomics Consortium (IAMDGC); Souzeau, Emmanuelle; Heid, I M; Weber, B H F (BioMed Central, 2017-03-27)
      Background Age-related macular degeneration (AMD) is a common condition of vision loss with disease development strongly influenced by environmental and genetic factors. Recently, 34 loci were associated with AMD at ...
    • Genetic polymorphism of the methotrexate transporter ABCG2, blood pressure and markers of arterial function in patients with rheumatoid arthritis: repeated cross-sectional study 

      Baghdadi, Leena R; Woodman, Richard John; Shanahan, Ernst Michael; Wiese, Michael D; Mangoni, Arduino Aleksander (Dove Press, 2018-11-12)
      Purpose: Methotrexate (MTX) treatment is associated with lower blood pressure (BP) and arterial stiffness in rheumatoid arthritis (RA). We investigated associations between single-nucleotide polymorphism (SNP) of the ...
    • Genetic study of Diabetic Retinopathy: recruitment methodology and analysis of baseline characteristics 

      Kaidonis, Georgia; Abhary, Sotoodeh; Daniell, Mark; Gillies, Mark; Fogarty, Rhys Daniel; Petrovsky, Nikolai; Jenkins, Alicia; Essex, Rohan; Chang, John H; Pal, Bishwanath; Hewitt, Alex W; Burdon, Kathryn Penelope; Craig, Jamie E (Wiley-Blackwell, 2013-09-30)
      BACKGROUND: Diabetic retinopathy (DR) is a blinding disease of increasing prevalence, caused by a complex interplay of genetic and environmental factors. Here we describe the patient recruitment methodology, case and control ...
    • Genome-wide association study identifies susceptibility loci for open angle glaucoma at TMCO1 and CDKN2B-AS1 

      Burdon, Kathryn Penelope; MacGregor, Stuart; Hewitt, Alex W; Sharma, Shiwani; Chidlow, Glyn; Mills, Richard Arthur; Danoy, Patrick; Casson, Robert J; Viswanathan, Ananth C; Liu, Jimmy Z; Landers, John; Henders, Anjali K; Wood, John; Souzeau, Emmanuelle; Crawford, April; Leo, Paul; Wang, Jie Jin; Nyholt, Dale R; Martin, Nicholas G; Montgomery, Grant W; Mitchell, Paul; Brown, Matthew A; Mackey, David A; Craig, Jamie E (Nature Publishing Group, 2011)
      A genome-wide association study (GWAS) for open angle glaucoma (OAG) blindness was conducted using a discovery cohort of 590 cases with severe visual field loss and 3956 controls. Genome-wide significant associations were ...
    • Genome-wide association study of intraocular pressure uncovers new pathways to glaucoma 

      MacGregor, Stuart; Ong, Jue Sheng; An, Jiyuan; Han, Xikun; Zhou, Tiger; Siggs, Owen M; Law, Matthew H; Souzeau, Emmanuelle; Sharma, Shiwani; Lynn, David J; Beesley, Jonathan; Sheldrick, Bronwyn; Mills, Richard Arthur; Landers, John; Ruddle, Jonathan B; Graham, Stuart L; Healey, Paul R; White, Andrew J; Casson, Robert J; Best, Stephen; Grigg, John RB; Goldberg, Ivan; Powell, Joseph E; Whiteman, David C; Smith, Graham; Martin, Nicholas G; Montgomery, Grant W; Burdon, Kathryn Penelope; Mackey, David A; Gharahkhani, Puya; Craig, Jamie E; Hewitt, Alex W (Springer Nature, 2018-07-27)
      Intraocular pressure (IOP) is currently the sole modifiable risk factor for primary open-angle glaucoma (POAG), one of the leading causes of blindness worldwide. Both IOP and POAG are highly heritable. We report a combined ...
    • Genome-wide meta-analyses of multiancestry cohorts identify multiple new susceptibility loci for refractive error and myopia 

      Verhoeven, Virginie J M; Hysi, Pirro G; Wojciechowski, Robert; Fan, Qiao; Guggenheim, Jeremy A; Hohn, Rene; MacGregor, Stuart; Hewitt, Alex W; Nag, Abhishek; Cheng, Ching-Yu; Yonova-Doing, Ekaterina; Zhou, Xin (National University of Singapore); Ikram, M Kamran; Buitendijk, Gabrielle H S; McMahon, George; Kemp, John P; St Pourcain, St Pourcain; Simpson, Claire L; Mäkela, Kari-Matti; Lehtimaki, Terho; Kahonen, Mika; Paterson, Andrew D; Hosseini, S Mohsen; Wong, Hoi Suen; Xu, Liang; Jonas, Jost B; Pärssinen, Olavi; Wedenoja, Juho; Yip, Shea Yip; Ho, Daniel W H; Pang, Chi Pui; Chen, Li-Jia; Klein, Barbara E K; Klein, Ronald; Haller, Toomas; Metspalu, Andres; Khor, Chiea Chuen; Tai, E-Shyong; Aung, Tin; Vithana, Eranga; Tay, Wan-Ting; Barathi, Veluchamy A; Chen, Peng; Li, Ruoying; Liao, Jiemin; Zheng, Yingfeng (Singapore Eye Research Institute); Ong, Rick T; Döring, Angela; Evans, David M; Timpson, Nicholas J; Verkerk, Annemieke J M H; Meitinger, Thomas; Raitakari, Olli; Hawthorne, Felicia; Spector, Tim D; Karssen, Lennart C; Pirastu, Mario; Murgia, Federico; Ang, Wei; Mishra, Aniket; Montgomery, Grant W; Pennell, Craig E; Cumberland, Phillippa M; Cotlarciuc, Ioana; Mitchell, Paul; Wang, Jie Jin; Schache, Maria; Janmahasathian, Sarayut; Igo Jr, Robert P; Lass, Jonathan H; Chew, Emily; Iyengar, Sudha K; Gorgels, Theo G M F; Rudan, Igor; Hayward, Caroline; Wright, Alan F; Craig, Jamie E; Burdon, Kathryn Penelope (Nature Publishing Group, 2013-02-10)
      Refractive error is the most common eye disorder worldwide and is a prominent cause of blindness. Myopia affects over 30% of Western populations and up to 80% of Asians. The CREAM consortium conducted genome-wide meta-analyses, ...
    • Glaucoma spectrum and age-related prevalence of individuals with FOXC1 and PITX2 variants 

      Souzeau, Emmanuelle; Siggs, Owen M; Zhou, Tiger; Galanopoulos, Anna; Hodson, Trevor; Taranath, Deepa A; Mills, Richard Arthur; Landers, John; Pater, John Brian; Smith, James E; Elder, James E; Rait, Julian L; Giles, Paul; Phakey, Vivek; Staffieri, Sandra E; Kearns, Lisa S; Dubowsky, Andrew; Mackey, David A; Hewitt, Alex W; Ruddle, Jonathan B; Burdon, Kathryn Penelope; Craig, Jamie E (Nature Publishing Group, 2017-05-17)
      Variation in FOXC1 and PITX2 is associated with Axenfeld-Rieger syndrome, characterised by structural defects of the anterior chamber of the eye and a range of systemic features. Approximately half of all affected individuals ...
    • Global causes of blindness and distance vision impairment 1990–2020: a systematic review and meta-analysis 

      Flaxman, Seth R; Bourne, Rupert R A; Resnikoff, Serge; Ackland, Peter; Braithwaite, Tasanee; Cicinelli, Maria V; Das, Aditi; Jonas, Jost B; Keeffe, Jill E; Kempen, John H; Leasher, Janet; Limburg, Hans; Naidoo, Kovin; Pesudovs, Konrad; Silvester, Alex; Stevens, Gretchen A; Tahhan, Nina; Wong, Tien Yin; Taylor, Hugh R; Vision Loss Expert Group of the Global Burden of Disease Study (Elsevier, 2017-10-11)
      Background Contemporary data for causes of vision impairment and blindness form an important basis of recommendations in public health policies. Refreshment of the Global Vision Database with recently published data sources ...
    • Global, regional, and national comparative risk assessment of 79 behavioural, environmental and occupational, and metabolic risks or clusters of risks, 1990–2015: a systematic analysis for the Global Burden of Disease Study 2015 

      Forouzanfar, Mohammad H; Afshin, Ashkan; Alexander, Lily T; Anderson, H Ross; Bhutta, Zulfiqar A; Biryukov, Stan; Brauer, Michael; Burnett, Richard; Cercy, Kelly; Charlson, Fiona J; Cohen, Aaron J; Dandona, Lalit; Estep, Kara; Ferrari, Alize J; Frostad, Joseph J; Fullman, Nancy; Gething, Peter W; Godwin, William W; Griswold, Max; Kinfu, Yohannes; Kyu, Hmwe Hmwe; Larson, Heidi J; Liang, Xiaofeng; Lim, Stephen S; Liu, Patrick Y; Pesudovs, Konrad; et. al. (Elsevier, 2016-10-08)
      Background The Global Burden of Diseases, Injuries, and Risk Factors Study 2015 provides an up-to-date synthesis of the evidence for risk factor exposure and the attributable burden of disease. By providing national and ...
    • Global, regional, and national disability-adjusted life-years (DALYs) for 315 diseases and injuries and healthy life expectancy (HALE), 1990–2015: a systematic analysis for the Global Burden of Disease Study 2015 

      Kassebaum, Nicholas J; Arora, Megha; Barber, Ryan M; Bhutta, Zulfiqar A; Brown, Jonathan; Carter, Austin; Casey, Daniel C; Charlson, Fiona J; Coates, Michael M; Coggeshall, Megan; Cornaby, Leslie; Dandona, Lalit; Dicker, Daniel J; Erskine, Holly E; Ferrari, Alize J; Fitzmaurice, Christina; Foreman, Kyle; Forouzanfar, Mohammad H; Fullman, Nancy; Gething, Peter W; Goldberg, Elkhonon; Graetz, Nicholas; Haagsma, Juanita A; Johnson, Catherine O; Kemmer, Laura; Pesudovs, Konrad; et. al. (Elsevier, 2016-10-08)
      Background Healthy life expectancy (HALE) and disability-adjusted life-years (DALYs) provide summary measures of health across geographies and time that can inform assessments of epidemiological patterns and health system ...
    • Global, regional, and national incidence, prevalence, and years lived with disability for 310 diseases and injuries, 1990–2015: a systematic analysis for the Global Burden of Disease Study 2015 

      Vos, Theo; Allen, Christine; Arora, Megha; Barber, Ryan M; Bhutta, Zulfiqar A; Brown, Alexandria; Carter, Austin; Casey, Daniel C; Charlson, Fiona J; Chen, Alan Z; Coggeshall, Megan; Cornaby, Leslie; Dandona, Lalit; Dicker, Daniel J; Dilegge, Tina; Erskine, Holly E; Ferrari, Alize J; Fitzmaurice, Christina; Fleming, Tom; Forouzanfar, Mohammad H; Fullman, Nancy; Gething, Peter W; Goldberg, Ellen M; Graetz, Nicholas; Haagsma, Juanita A; Pesudovs, Konrad; et. al. (Elsevier, 2016-10-08)
      Background Non-fatal outcomes of disease and injury increasingly detract from the ability of the world's population to live in full health, a trend largely attributable to an epidemiological transition in many countries ...
    • Global, regional, and national levels of maternal mortality, 1990–2015: a systematic analysis for the Global Burden of Disease Study 2015 

      Kassebaum, Nicholas J; Barber, Ryan M; Bhutta, Zulfiqar A; Dandona, Lalit; Gething, Peter W; Hay, Simon I; Kinfu, Yohannes; Larson, Heidi J; Liang, Xiaofeng; Lim, Stephen S; Lopez, Alan D; Lozano, Rafael; Mensah, George A; Mokdad, Ali H; Naghavi, Mohsen; Pinho, Christine; Salomon, Joshua A; Steiner, Caitlyn; Vos, Theo; Wang, Haidong; Abajobir, Amanuel Alemu; Abate, Kalkidan Hassen; Abbas, Kaja M; Abd-Allah, Foad; Abdallat, Mahmud A; Pesudovs, Konrad; et. al. (Elsevier, 2016-10-08)
      Background In transitioning from the Millennium Development Goal to the Sustainable Development Goal era, it is imperative to comprehensively assess progress toward reducing maternal mortality to identify areas of success, ...
    • Global, regional, and national life expectancy, all-cause mortality, and cause-specific mortality for 249 causes of death, 1980–2015: a systematic analysis for the Global Burden of Disease Study 2015 

      Wang, Haidong; Naghavi, Mohsen; Allen, Christine; Barber, Ryan M; Bhutta, Zulfiqar A; Carter, Austin; Casey, Daniel C; Charlson, Fiona J; Chen, Alan Z; Coates, Michael M; Coggeshall, Megan; Dandona, Lalit; Dicker, Daniel J; Erskine, Holly E; Ferrari, Alize J; Fitzmaurice, Christina; Foreman, Kyle; Forouzanfar, Mohammad H; Fraser, Maya S; Fullman, Nancy; Gething, Peter W; Goldberg, Ellen M; Graetz, Nicholas; Haagsma, Juanita A; Hay, Simon I; Pesudovs, Konrad; et. al. (Elsevier, 2016-10-08)
      Background Improving survival and extending the longevity of life for all populations requires timely, robust evidence on local mortality levels and trends. The Global Burden of Disease 2015 Study (GBD 2015) provides a ...
    • Global, regional, national, and selected subnational levels of stillbirths, neonatal, infant, and under-5 mortality, 1980–2015: a systematic analysis for the Global Burden of Disease Study 2015 

      Wang, Haidong; Bhutta, Zulfiqar A; Coates, Matthew M; Coggeshall, Megan; Dandona, Lalit; Diallo, Khassoum; Franca, Elisabeth Barboza; Fraser, Maya; Fullman, Nancy; Gething, Peter W; Hay, Simon I; Kinfu, Yohannes; Kita, Maaya; Kulikoff, Xie Rachel; Larson, Heidi J; Liang, Juan; Liang, Xiaofeng; Lind, Margaret; Lopez, Alan D; Lozano, Rafael; Mensah, George A; Mikesell, Joseph B; Mokdad, Ali H; Mooney, Meghan D; Nguyen, Grant; Pesudovs, Konrad; et. al. (Elsevier, 2016-10-08)
      Background Established in 2000, Millennium Development Goal 4 (MDG4) catalysed extraordinary political, financial, and social commitments to reduce under-5 mortality by two-thirds between 1990 and 2015. At the country ...
    • GWAS study using DNA pooling strategy identifies association of variant rs4910623 in OR52B4 gene with anti-VEGF treatment response in age-related macular degeneration 

      Riaz, Moeen; Lores-Motta, Laura; Richardson, Andrea J; Lu, Yi; Montgomery, Grant W; Omar, Amer; Koenekoop, Robert K; Chen, John; Muether, Philipp; Altay, Lebriz; Schick, Tina; Fauser, Sascha; Smailhodzic, Dzenita; van Asten, Freekje; de Jong, Eiko K; Hoyng, Carel B; Burdon, Kathryn Penelope; MacGregor, Stuart; Guymer, Robyn H; den Hollander, Anneke I; Baird, Paul N (Nature Publishing Group, 2016-11-28)
      Pooled DNA based GWAS to determine genetic association of SNPs with visual acuity (VA) outcome in anti-vascular endothelial growth factor (anti-VEGF) treated neovascular age-related macular degeneration (nAMD) patients. ...
    • High throughput genetic screening of 51 paediatric cataract genes identifies causative mutations in inherited paediatric cataract in South Eastern Australia 

      Javadiyan, Shahrbanou; Craig, Jamie E; Souzeau, Emmanuelle; Sharma, Shiwani; Lower, Karen Marie; Mackey, David A; Staffieri, Sandra E; Elder, James E; Taranath, Deepa A; Straga, Tania; Black, Joanna; Pater, John Brian; Casey, Theresa; Hewitt, Alex W; Burdon, Kathryn Penelope (Genetics Society of America., 2017-08-24)
      Paediatric cataract is a leading cause of childhood blindness. This study aimed to determine the genetic cause of paediatric cataract in Australian families by screening known disease associated genes using massively ...