Show simple item record

dc.contributor.authorKitchener, Andrew C.
dc.contributor.authorLinacre, Adrian Matthew Thornton
dc.contributor.authorTobe, Shanan S
dc.date.accessioned2014-09-30T06:05:38Z
dc.date.available2014-09-30T06:05:38Z
dc.date.issued2010en_US
dc.identifier.citationTobe, S.S., Kitchener, A.C. and Linacre, A.M.T. (2010). Reconstructing Mammalian Phylogenies: A Detailed Comparison of the Cytochrome b and Cytochrome Oxidase Subunit I Mitochondrial Genes. PLoS One, 5 pp. e14156.en
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/2328/32040
dc.descriptionCopyright: © 2010 Tobe et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.description.abstractThe phylogeny and taxonomy of mammalian species were originally based upon shared or derived morphological characteristics. However, genetic analyses have more recently played an increasingly important role in confirming existing or establishing often radically different mammalian groupings and phylogenies. The two most commonly used genetic loci in species identification are the cytochrome oxidase I gene (COI) and the cytochrome b gene (cyt b). For the first time this study provides a detailed comparison of the effectiveness of these two loci in reconstructing the phylogeny of mammals at different levels of the taxonomic hierarchy in order to provide a basis for standardizing methodologies in the future. Interspecific and intraspecific variation is assessed and for the first time, to our knowledge, statistical confidence is applied to sequence comparisons. Comparison of the DNA sequences of 217 mammalian species reveals that cyt b more accurately reconstructs their phylogeny and known relationships between species based on other molecular and morphological analyses at Super Order, Order, Family and generic levels. Cyt b correctly assigned 95.85% of mammal species to Super Order, 94.31% to Order and 98.16% to Family compared to 78.34%, 93.36% and 96.93% respectively for COI. Cyt b also gives better resolution when separating species based on sequence data. Using a Kimura 2-parameter p-distance (x100) threshold of 1.5–2.5, cyt b gives a better resolution for separating species with a lower false positive rate and higher positive predictive value than those of COI.en
dc.language.isoen
dc.rightsCopyright: © 2010 Tobe et al.en
dc.titleReconstructing Mammalian Phylogenies: A Detailed Comparison of the Cytochrome b and Cytochrome Oxidase Subunit I Mitochondrial Genesen
dc.typeArticleen
dc.identifier.rmid2006019696
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0014156en
dc.subject.forgroup0699 Other Biological Sciencesen
dc.rights.holderTobe et al.en
dc.rights.licenseCC-BY
local.contributor.authorOrcidLookupLinacre, Adrian Matthew Thornton: https://orcid.org/0000-0001-5890-5548en_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record