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dc.contributor.authorTrijatmiko, Kurniawan R
dc.contributor.authorDuenas, Conrado
dc.contributor.authorTsakirpaloglou, Nikolaos
dc.contributor.authorTorrizo, Lina
dc.contributor.authorArines, Felichi Mae
dc.contributor.authorAdeva, Cheryl
dc.contributor.authorBalindong, Jeanette
dc.contributor.authorOliva, Norman
dc.contributor.authorSapasap, Maria V
dc.contributor.authorBorrero, Jaime
dc.contributor.authorRey, Jessica
dc.contributor.authorFrancisco, Perigio
dc.contributor.authorNelson, Andy
dc.contributor.authorNakanishi, Hiromi
dc.contributor.authorLombi, Enzo
dc.contributor.authorTako, Elad
dc.contributor.authorGlahn, Raymond P
dc.contributor.authorStangoulis, James Constantine Roy
dc.contributor.authorChadha-Mohanty, Prabhjit
dc.contributor.authorJohnson, Alexander A T
dc.contributor.authorTohme, Joe
dc.contributor.authorBarry, Gerard
dc.contributor.authorSlamet-Loedin, Inez H
dc.date.accessioned2016-05-31T02:02:24Z
dc.date.available2016-05-31T02:02:24Z
dc.date.issued2016
dc.identifier.citationTrijatmiko, K. R. et al. Biofortified indica rice attains iron and zinc nutrition dietary targets in the field. Sci. Rep. 6, 19792; doi: 10.1038/srep19792 (2016).en
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/2328/36155
dc.descriptionThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.description.abstractMore than two billion people are micronutrient deficient. Polished grains of popular rice varieties have concentration of approximately 2 μg g−1 iron (Fe) and 16 μg g−1 zinc (Zn). The HarvestPlus breeding programs for biofortified rice target 13 μg g−1 Fe and 28 μg g−1 Zn to reach approximately 30% of the estimated average requirement (EAR). Reports on engineering Fe content in rice have shown an increase up to 18 μg g−1 in glasshouse settings; in contrast, under field conditions, 4 μg g−1 was the highest reported concentration. Here, we report on selected transgenic events, field evaluated in two countries, showing 15 μg g−1 Fe and 45.7 μg g−1 Zn in polished grain. Rigorous selection was applied to 1,689 IR64 transgenic events for insert cleanliness and, trait and agronomic performances. Event NASFer-274 containing rice nicotianamine synthase (OsNAS2) and soybean ferritin (SferH-1) genes showed a single locus insertion without a yield penalty or altered grain quality. Endosperm Fe and Zn enrichment was visualized by X-ray fluorescence imaging. The Caco-2 cell assay indicated that Fe is bioavailable. No harmful heavy metals were detected in the grain. The trait remained stable in different genotype backgrounds.en
dc.language.isoen
dc.publisherNature Publishing Groupen
dc.rightsCopyright 2016 The Authorsen
dc.titleBiofortified indica rice attains iron and zinc nutrition dietary targets in the fielden
dc.typeArticleen
dc.identifier.doihttps://doi.org/10.1038/srep19792en
dc.rights.holderThe Authorsen
local.contributor.authorOrcidLookupStangoulis, James Constantine Roy: https://orcid.org/0000-0001-7778-7110en_US


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