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dc.contributor.authorYeadon, Patricia Jane
dc.contributor.authorBowring, Frederick James
dc.contributor.authorCatcheside, David Edward
dc.date.accessioned2016-05-31T05:23:05Z
dc.date.available2016-05-31T05:23:05Z
dc.date.issued2016
dc.identifier.citationYeadon PJ, Bowring FJ, Catcheside DEA (2016) Meiotic Recombination in Neurospora crassa Proceeds by Two Pathways with Extensive Holliday Junction Migration. PLoS ONE 11(1): e0147815. doi:10.1371/journal.pone.0147815en
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/2328/36159
dc.descriptionThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.description.abstractAnalysis of thousands of Δmsh-2 octads using our fluorescent recombination system indicates that, as in other filamentous fungi, symmetric heteroduplex is common in the his-3 region of Neurospora crassa. Symmetric heteroduplex arises from Holliday junction migration, and we suggest this mechanism explains the high frequency of His+ spores in heteroallelic crosses in which recombination is initiated cis to the his-3 allele further from the initiator, cog+. In contrast, when recombination is initiated cis to the his-3 allele closer to cog+, His+ spores are mainly a result of synthesis-dependent strand annealing, yielding asymmetric heteroduplex. Loss of Msh-2 function increases measures of allelic recombination in both his-3 and the fluorescent marker gene, indicating that mismatches in asymmetric heteroduplex, as in Saccharomyces cerevisiae, tend to be repaired in the direction of restoration. Furthermore, the presence of substantial numbers of conversion octads in crosses lacking Msh-2 function suggests that the disjunction pathway described in S. cerevisiae is also active in Neurospora, adding to evidence for a universal model for meiotic recombination.en
dc.language.isoen
dc.publisherPublic Library of Scienceen
dc.rightsCopyrigh: © 2016 Yeadon et al.en
dc.titleMeiotic Recombination in Neurospora crassa Proceeds by Two Pathways with Extensive Holliday Junction Migrationen
dc.typeArticleen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0147815en
dc.rights.holderThe Authorsen
dc.rights.licenseCC-BY
local.contributor.authorOrcidLookupCatcheside, David: https://orcid.org/0000-0002-7101-6849en_US


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