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dc.contributor.authorPalmer, Gwen
dc.contributor.authorHibberd, Timothy J
dc.contributor.authorRoose, Tiina
dc.contributor.authorBrookes, Simon Jonathan
dc.contributor.authorTaylor, Mark
dc.date.accessioned2017-03-03T05:24:49Z
dc.date.available2017-03-03T05:24:49Z
dc.date.issued2016-11-01
dc.identifier.citationPalmer G, Hibberd TJ, Roose T, Brookes SJ, Taylor M. Measurement of strains experienced by viscerofugal nerve cell bodies during mechanosensitive firing using digital image correlation. Am J Physiol Gastrointest Liver Physiol. 2016 Nov 1;311(5):G869-G879.en
dc.identifier.urihttp://hdl.handle.net/2328/36940
dc.description12 month embargo until 1 Nov 2017, as per publisher's policy.en
dc.description.abstractMechanosensory neurons detect physical events in the local environments of the tissues that they innervate. Studies of mechanosensitivity of neurons or nerve endings in the gut have related their firing to strain, wall tension or pressure. Digital Image Correlation (DIC) is a technique from materials engineering that can be adapted to measure the local physical environments of afferent neurons at high resolution. Flat sheet preparations of guinea pig distal colon were set up with arrays of tissue markers, in vitro. Firing of single viscerofugal neurons was identified in extracellular colonic nerve recordings. The locations of viscerofugal nerve cell bodies were inferred by mapping firing responses to focal application of the nicotinic receptor agonist, DMPP. Mechanosensory firing was recorded during load-evoked uni-axial or bi-axial distensions. Distension caused movement of surface markers which was captured using video imaging. DIC tracked the markers, interpolating the mechanical state of the gut at the location of the viscerofugal nerve cell body. This technique revealed heterogeneous load-evoked strain within preparations. Local strains at viscerofugal nerve cell bodies were usually smaller than global strain measurements and correlated more closely with mechanosensitive firing. Both circumferential and longitudinal strain activated viscerofugal neurons. Simultaneous loading in circumferential and longitudinal axes, caused the highest levels of viscerofugal neuron firing. Multiaxial strains, reflecting tissue shearing and changing area, linearly correlated with mechanosensory firing of viscerofugal neurons. Viscerofugal neurons were mechanically sensitive to both local circumferential and local longitudinal gut strain, and appear to lack directionality in their stretch sensitivity.en
dc.language.isoenen
dc.publisherThe American Physiological Society.en
dc.relationhttp://purl.org/au-research/grants/nhmrc/1048195en
dc.subjectMechanosensoryen
dc.subjectAfferenten
dc.subjectStrainen
dc.subjectBiomechanicsen
dc.subjectViscerofugalen
dc.subjectDigital image correlationen
dc.titleMeasurement of strains experienced by viscerofugal nerve cell bodies during mechanosensitive firing using digital image correlation.en
dc.typeArticleen
dc.relation.grantnumberNHMRC/1048195en
dc.identifier.doihttps://doi.org/10.1152/ajpgi.00397.2015en
dc.rights.holderCopyright © 2016 the American Physiological Society.en
dc.rights.licenseIn Copyright
local.contributor.authorOrcidLookupBrookes, Simon Jonathan: https://orcid.org/0000-0001-5635-0876en_US
local.contributor.authorOrcidLookupHibberd, Timothy J: https://orcid.org/0000-0002-3747-140Xen_US
local.contributor.authorOrcidLookupTaylor, Mark: https://orcid.org/0000-0001-7842-6472en_US


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