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    Microbial cell lysis and nucleic acid extraction via nanofluidic channel

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    Date
    2015-03-04
    Author
    Kant, Krishna
    Yoo, J
    Amos, S
    Erkelens, M
    Priest, C
    Shapter, Joseph George
    Losic, D
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    Abstract
    This paper presents a microfluidic device with a nano-channel prepared by focused ion beam (FIB) milling for microbial cell lysis and nucleic acid extraction. The device prepared in a quartz slide combines two microchannels connected with one nanochannel in the middle with dimensions of 100 nm (width) and 250 nm (depth) and 1 μm (length). The capturing of a single or a few cells and their lysis for extraction of DNA in nanochannel were demonstrated. The purity and quantity of the extracted genetic material was tested using a UV spectrometer. The results revealed that the extraction methodology was successful and may be well-suited to integration in analytical lab-on-a-chip devices.
    URI
    http://hdl.handle.net/2328/37158
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    • Chemistry, Physics and Earth Sciences - Collected Works
    • Flinders Open Access Research

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