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dc.contributor.authorChoo, Jocelyn M
dc.contributor.authorTrim, Paul J
dc.contributor.authorLeong, Lex E X
dc.contributor.authorAbell, Guy
dc.contributor.authorBrune, Carly
dc.contributor.authorJefferies, Nicole
dc.contributor.authorWesselingh, Steven L
dc.contributor.authorDear, T Neil
dc.contributor.authorSnel, Marten F
dc.contributor.authorRogers, Geraint B
dc.date.accessioned2017-06-05T23:56:08Z
dc.date.available2017-06-05T23:56:08Z
dc.date.issued2017-04-11
dc.identifier.citationChoo JM, Trim PJ, Leong LEX, Abell GCJ, Brune C, Jeffries N, Wesselingh S, Dear TN, Snel MF and Rogers GB (2017) Inbred Mouse Populations Exhibit Intergenerational Changes in Intestinal Microbiota Composition and Function Following Introduction to a Facility. Front. Microbiol. 8:608. doi: 10.3389/fmicb.2017.00608en
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/2328/37260
dc.descriptionThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.description.abstractInbred mice are used to investigate many aspects of human physiology, including susceptibility to disease and response to therapies. Despite increasing evidence that the composition and function of the murine intestinal microbiota can substantially influence a broad range of experimental outcomes, relatively little is known about microbiome dynamics within experimental mouse populations. We investigated changes in the intestinal microbiome between C57BL/6J mice spanning six generations (assessed at generations 1, 2, 3, and 6), following their introduction to a stringently controlled facility. Fecal microbiota composition and function were assessed by 16S rRNA gene amplicon sequencing and liquid chromatography mass spectrometry, respectively. Significant divergence of the intestinal microbiota between founder and second generation mice, as well as continuing inter-generational variance, was observed. Bacterial taxa whose relative abundance changed significantly through time included Akkermansia, Turicibacter, and Bifidobacterium (p < 0.05), all of which are recognized as having the potential to substantially influence host physiology. Shifts in microbiota composition were mirrored by corresponding differences in the fecal metabolome (r = 0.57, p = 0.0001), with notable differences in levels of tryptophan pathway metabolites and amino acids, including glutamine, glutamate and aspartate. We related the magnitude of changes in the intestinal microbiota and metabolome characteristics during acclimation to those observed between populations housed in separate facilities, which differed in regards to husbandry, barrier conditions and dietary intake. The microbiome variance reported here has implications for experimental reproducibility, and as a consequence, experimental design and the interpretation of research outcomes across wide range of contexts.en
dc.language.isoen
dc.publisherFrontiers Media S.A.en
dc.rightsCopyright © 2017 Choo, Trim, Leong, Abell, Brune, Jeffries, Wesselingh, Dear, Snel and Rogers.en
dc.subjectC57BL/6Jen
dc.subjectinbred miceen
dc.subjectmice generationsen
dc.subjectfecal microbiotaen
dc.subjectfecal metabolomeen
dc.subjectmicrobiome variationen
dc.titleInbred Mouse Populations Exhibit Intergenerational Changes in Intestinal Microbiota Composition and Function Following Introduction to a Facilityen
dc.typeArticleen
dc.identifier.doihttps://doi.org/10.3389/fmicb.2017.00608en
dc.rights.holderChoo, Trim, Leong, Abell, Brune, Jeffries, Wesselingh, Dear, Snel and Rogers.en


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