Show simple item record

dc.contributor.authorChoo, Jocelyn M
dc.contributor.authorAbell, Guy C J
dc.contributor.authorThomson, Rachel
dc.contributor.authorMorgan, Lucy
dc.contributor.authorWaterer, Grant
dc.contributor.authorGordon, David Llewellyn
dc.contributor.authorTaylor, Steven L
dc.contributor.authorLeong, Lex E X
dc.contributor.authorWesselingh, Steven L
dc.contributor.authorBurr, Lucy D
dc.contributor.authorRogers, Geraint B
dc.date.accessioned2018-08-29T23:50:18Z
dc.date.available2018-08-29T23:50:18Z
dc.date.issued2018-04-18
dc.identifier.citationChoo, J. M., Abell, G. C. J., Thomson, R., Morgan, L., Waterer, G., Gordon, D. L., Taylor, S. L., Leong, L. E. X., Wesselingh, S. L., Burr, L. D. & Rogers, G. B., (2018). Impact of Long-Term Erythromycin Therapy on the Oropharyngeal Microbiome and Resistance Gene Reservoir in Non-Cystic Fibrosis Bronchiectasis. mSphere, 3(2): 1-18.en_US
dc.identifier.issn2379-5042
dc.identifier.urihttp://hdl.handle.net/2328/38218
dc.descriptionThis is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International license.en_US
dc.description.abstractLong-term macrolide therapy reduces rates of pulmonary exacerbation in bronchiectasis. However, little is known about the potential for macrolide therapy to alter the composition and function of the oropharyngeal commensal microbiota or to increase the carriage of transmissible antimicrobial resistance. We assessed the effect of long-term erythromycin on oropharyngeal microbiota composition and the carriage of transmissible macrolide resistance genes in 84 adults with bronchiectasis, enrolled in the Bronchiectasis and Low-dose Erythromycin Study (BLESS) 48-week placebo-controlled trial of twice-daily erythromycin ethylsuccinate (400 mg). Oropharyngeal microbiota composition and macrolide resistance gene carriage were determined by 16S rRNA gene amplicon sequencing and quantitative PCR, respectively. Long-term erythromycin treatment was associated with a significant increase in the relative abundance of oropharyngeal Haemophilus parainfluenzae (P = 0.041) and with significant decreases in the relative abundances of Streptococcus pseudopneumoniae (P = 0.024) and Actinomyces odontolyticus (P = 0.027). Validation of the sequencing results by quantitative PCR confirmed a significant decrease in the abundance of Actinomyces spp. (P = 0.046). Erythromycin treatment did not result in a significant increase in the number of subjects who carried erm(A), erm(B), erm(C), erm(F), mef(A/E), and msrA macrolide resistance genes. However, the abundance of erm(B) and mef(A/E) gene copies within carriers who had received erythromycin increased significantly (P < 0.05). Our findings indicate that changes in oropharyngeal microbiota composition resulting from long-term erythromycin treatment are modest and are limited to a discrete group of taxa. Associated increases in levels of transmissible antibiotic resistance genes within the oropharyngeal microbiota highlight the potential for this microbial system to act as a reservoir for resistance.en_US
dc.description.sponsorshipThis study was funded by the National Health and Medical Research Council of Australia (NHMRC project identifier 11044000).en_US
dc.language.isoenen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.rights© Crown copyright 2018. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.en_US
dc.subjectantibiotic resistanceen_US
dc.subjectbronchiectasisen_US
dc.subjectmacrolide therapyen_US
dc.subjectoropharyngeal microbiomeen_US
dc.titleImpact of Long-Term Erythromycin Therapy on the Oropharyngeal Microbiome and Resistance Gene Reservoir in Non-Cystic Fibrosis Bronchiectasisen_US
dc.typeArticleen
dc.relation.grantnumberNHMRC/11044000en_US
dc.identifier.doihttps://doi.org/10.1128/mSphere.00103-18en
dc.rights.holder© Crown copyright 2018.en_US
local.contributor.authorOrcidLookupGordon, David Llewellyn: https://orcid.org/0000-0003-3276-9685en_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record