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dc.contributor.authorHugouvieux, Veronique
dc.contributor.authorSilva, Catarina S
dc.contributor.authorJourdain, Agnes
dc.contributor.authorStigliani, Arnaud
dc.contributor.authorCharras, Quentin
dc.contributor.authorConn, Vanessa
dc.contributor.authorConn, Simon
dc.contributor.authorCarles, Cristel C
dc.contributor.authorParcy, Francois
dc.contributor.authorZubieta, Chloe
dc.date.accessioned2018-08-30T03:20:22Z
dc.date.available2018-08-30T03:20:22Z
dc.date.issued2018-03-19
dc.identifier.citationHugouvieux, V., Silva, C. S., Jourdain, A., Stigliani, A., Charras, Q., Conn, V., Conn, S., Carles, C. C., Parcy, F. & Zubieta, C., (2018). Tetramerization of MADS family transcription factors SEPALLATA3 and AGAMOUS is required for floral meristem determinacy in Arabidopsis. Nucleic Acids Research, 46(10): 4966-4977.en_US
dc.identifier.issn0305-1048
dc.identifier.urihttp://hdl.handle.net/2328/38224
dc.descriptionPublished by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.comen_US
dc.description.abstractThe MADS transcription factors (TF) constitute an ancient family of TF found in all eukaryotes that bind DNA as obligate dimers. Plants have dramatically expanded the functional diversity of the MADS family during evolution by adding protein–protein interaction domains to the core DNA-binding domain, allowing the formation of heterotetrameric complexes. Tetramerization of plant MADS TFs is believed to play a central role in the evolution of higher plants by acting as one of the main determinants of flower formation and floral organ specification. The MADS TF, SEPALLATA3 (SEP3), functions as a central protein–protein interaction hub, driving tetramerization with other MADS TFs. Here, we use a SEP3 splice variant, SEP3Δtet, which has dramatically abrogated tetramerization capacity to decouple SEP3 tetramerization and DNA-binding activities. We unexpectedly demonstrate that SEP3 heterotetramer formation is required for correct termination of the floral meristem, but plays a lesser role in floral organogenesis. The heterotetramer formed by SEP3 and the MADS protein, AGAMOUS, is necessary to activate two target genes, KNUCKLES and CRABSCLAW, which are required for meristem determinacy. These studies reveal unique and highly specific roles of tetramerization in flower development and suggest tetramerization may be required to activate only a subset of target genes in closed chromatin regions.en_US
dc.description.sponsorshipAction Thematique et Incitative sur Programme (ATIP)- Avenir (to C.Z.); Grenoble Alliance for Integrated Structural Cell Biology (ANR-10-LABX-49-01 to V.H., A.J., C.Z., Q.C., A.S., V.C., F.P.); Agence Nationale de la Recherche (project Flopinet to C.Z., V.H., A.J., F.P., C.C.) and Centre National de la Recherche Scientifique (to S.J.C.). Funding for open access charge: ATIP-Avenir, ANR.en_US
dc.language.isoenen_US
dc.publisherOxford University Pressen_US
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.comen_US
dc.subjectgene regulationen_US
dc.subjectchromatinen_US
dc.subjectepigeneticsen_US
dc.titleTetramerization of MADS family transcription factors SEPALLATA3 and AGAMOUS is required for floral meristem determinacy in Arabidopsisen_US
dc.typeArticleen
dc.identifier.doihttps://doi.org/10.1093/nar/gky205en
dc.rights.holder©The Author(s) 2018.en_US
dc.rights.licenseCC-BY-NC


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