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dc.contributor.authorZotova, Lyudmila
dc.contributor.authorKurishbayev, Akhylbek
dc.contributor.authorJatayev, Satyvaldy
dc.contributor.authorKhassanova, Gulmira
dc.contributor.authorZhubatkanov, Askar
dc.contributor.authorSerikbay, Dauren
dc.contributor.authorSereda, Sergey
dc.contributor.authorSereda, Tatiana
dc.contributor.authorShvidchenko, Vladimir
dc.contributor.authorLopato, Sergiy
dc.contributor.authorJenkins, Colin L D
dc.contributor.authorSoole, Kathleen Lydia
dc.contributor.authorLangridge, Peter
dc.contributor.authorShavrukov, Yuri
dc.date.accessioned2018-10-18T01:14:55Z
dc.date.available2018-10-18T01:14:55Z
dc.date.issued2018-09-27
dc.identifier.citationZotova, L., Kurishbayev, A., Jatayev ,S. et al., (2018). Genes Encoding Transcription Factors TaDREB5 and TaNFYC-A7 Are Differentially Expressed in Leaves of Bread Wheat in Response to Drought, Dehydration and ABA. Front. Plant Sci. 9:1441.en_US
dc.identifier.issn1664-462X
dc.identifier.urihttp://hdl.handle.net/2328/38426
dc.descriptionThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_US
dc.description.abstractTwo groups of six spring bread wheat varieties with either high or low grain yield under the dry conditions of Central and Northern Kazakhstan were selected for analysis. Experiments were set up with the selected wheat varieties in controlled environments as follows: (1) slowly progressing drought imposed on plants in soil, (2) rapid dehydration of whole plants grown in hydroponics, (3) dehydration of detached leaves, and (4) ABA treatment of whole plants grown in hydroponics. Representatives of two different families of transcription factors (TFs), TaDREB5 and TaNFYC-A7, were found to be linked to yield-under-drought using polymorphic Amplifluor-like SNP marker assays. qRT-PCR revealed differing patterns of expression of these genes in the leaves of plants subjected to the above treatments. Under drought, TaDREB5 was significantly up-regulated in leaves of all high-yielding varieties tested and down-regulated in all low-yielding varieties, and the level of expression was independent of treatment type. In contrast, TaNFYC-A7 expression levels showed different responses in the high- and low-yield groups of wheat varieties. TaNFYC-A7 expression under dehydration (treatments 2 and 3) was higher than under drought (treatment 1) in all high-yielding varieties tested, while in all low-yielding varieties the opposite pattern was observed: the expression levels of this gene under drought were higher than under dehydration. Rapid dehydration of detached leaves and intact wheat plants grown in hydroponics produced similar changes in gene expression. ABA treatment of whole plants caused rapid stomatal closure and a rise in the transcript level of both genes during the first 30 min, which decreased 6 h after treatment. At this time-point, expression of TaNFYC-A7 was again significantly up-regulated compared to untreated controls, while TaDREB5 returned to its initial level of expression. These findings reveal significant differences in the transcriptional regulation of two drought-responsive and ABA-dependent TFs under slowly developing drought and rapid dehydration of wheat plants. The results obtained suggest that correlation between grain yield in dry conditions and TaNFYC-A7 expression levels in the examined wheat varieties is dependent on the length of drought development and/or strength of drought; while in the case of TaDREB5, no such dependence is observed.en_US
dc.description.sponsorshipResearch program BR05236500 (SJ) has been supported by the Ministry of Education and Science (Kazakhstan), and ARC project DP170103975 (PL) entitled ‘Accessing diversity in large crop genomes via enhanced recombination’ (Australia).en_US
dc.language.isoenen_US
dc.publisherFrontiers Mediaen_US
dc.rightsCopyright © 2018 Zotova, Kurishbayev, Jatayev, Khassanova, Zhubatkanov, Serikbay, Sereda, Sereda, Shvidchenko, Lopato, Jenkins, Soole, Langridge and Shavrukov. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_US
dc.subjectABA treatmenten_US
dc.subjectdehydrationen_US
dc.subjectdroughten_US
dc.subjectgene expressionen_US
dc.subjectgrain yielden_US
dc.subjectqRT-PCRen_US
dc.subjectstomatal conductanceen_US
dc.subjectwheaten_US
dc.titleGenes Encoding Transcription Factors TaDREB5 and TaNFYC-A7 Are Differentially Expressed in Leaves of Bread Wheat in Response to Drought, Dehydration and ABAen_US
dc.typeArticleen_US
dc.relation.grantnumberARC/DP170103975en_US
dc.identifier.doihttps://doi.org/10.3389/fpls.2018.01441en_US
dc.rights.holderCopyright © 2018 Zotova, Kurishbayev, Jatayev, Khassanova, Zhubatkanov, Serikbay, Sereda, Sereda, Shvidchenko, Lopato, Jenkins, Soole, Langridge and Shavrukov.en_US
dc.rights.licenseCC-BY
local.contributor.authorOrcidLookupJenkins, Colin L D: https://orcid.org/0000-0002-9347-8948en_US
local.contributor.authorOrcidLookupShavrukov, Yuri: https://orcid.org/0000-0002-2941-0340en_US
local.contributor.authorOrcidLookupSoole, Kathleen Lydia: https://orcid.org/0000-0002-8837-3404en_US


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