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dc.contributor.authorCurrie, Bart John
dc.contributor.authorInglis, T J
dc.contributor.authorNorton, R
dc.contributor.authorRodrigues, F
dc.date.accessioned2010-07-27T05:53:55Z
dc.date.available2010-07-27T05:53:55Z
dc.date.issued2004en_US
dc.identifier.citationInglis, T.J., Rodrigues, F., Norton, R., & Currie, B.J., 2004. Comparison of the susceptibilities of burkholderia pseudomallei to meropenem and ceftazidime by conventional and intracellular methods. Antimicrobial Agents and Chemotherapy, 48(8), 2999-3005.en
dc.identifier.issn0066-4804
dc.identifier.urihttp://hdl.handle.net/2328/9343
dc.description.abstractThe effect of the two antibiotics ceftazidime and meropenem on a collection of 46 Burkholderia pseudomallei isolates representing clinical and environmental sources across northern Australia was investigated by using a series of in vitro test methods. The susceptibility testing methods used included Kirby-Bauer disk diffusion, Etest MIC, broth microdilution MIC, and a modification of the microdilution method in which Acanthamoeba cells were added to simulate the effect of a professional phagocytic cell on test outcome. In a semiquantitative validation coculture series, the majority of bacteria were intracellular up to a multiplicity of infection of 10 bacteria to one ameba. The optical density and bacterial count (log10 CFU/ml) correlated across the range tested (r2 0.77; P < 0.0001). Susceptibility test results were compared against clinical outcomes. The MICs of ceftazidime were consistently higher than those of meropenem by all three methods. The MICs of both agents were significantly higher when Acanthamoeba trophozoites were added to the broth microdilution method. Conventional and intracellular MIC results were consistent for clinical isolates from the Western Australian outbreak cluster despite the wide variety of clinical outcomes. Further development of the intracellular MIC method is expected to help assess the efficacy of antimicrobial agents on this bacterial species in an intracellular setting.en
dc.titleComparison of the susceptibilities of burkholderia pseudomallei to meropenem and ceftazidime by conventional and intracellular methodsen
dc.typeArticleen
dc.identifier.rmid'2004054245
dc.identifier.doihttps://doi.org/10.1128/AAC.48.8.2999-3005.2004en
dc.description.note1752 N ST NW, WASHINGTON, USA, DC, 20036-2904en
dc.subject.forgroup0605 Microbiologyen
dc.subject.forgroup1108 Medical Microbiologyen
dc.rights.licenseIn Copyright


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